› 4k33

X-ray diffraction
2.34Å resolution

Crystal Structure of FGF Receptor 3 (FGFR3) Kinase Domain Harboring the K650E Mutation, a Gain-of-Function Mutation Responsible for Thanatophoric Dysplasia Type II and Spermatocytic Seminoma

Released:
DOI: 10.2210/pdb4k33/pdb
PDB entry 4k33 coloured by chain, front view.
PDB entry 4k33 coloured by chain, side view.
PDB entry 4k33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mimicry of a-loop tyrosine phosphorylation by a pathogenic FGF receptor 3 mutation.
Huang Z, Chen H, Blais S, Neubert TA, Li X, Mohammadi M
Structure 21 1889-96 (2013)
PMID: 23972473

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor receptor 3 Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 36.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22607 (Residues: 449-759; Coverage: 40%)
Gene names: FGFR3, JTK4
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 54.226Å b: 61.427Å c: 100.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.176 0.228
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A threshold model for receptor tyrosine kinase signaling specificity and cell fate determination.
Zinkle et al. (2018)
2 more

19 mentions without citation

Structure, activation and dysregulation of fibroblast growth factor receptor kinases: perspectives for clinical targeting.
Farrell et al. (2018)
18 more