› 4k44

X-ray diffraction
1.7Å resolution

Auto-inhibition and phosphorylation-induced activation of PLC-gamma isozymes

Released:
DOI: 10.2210/pdb4k44/pdb
PDB entry 4k44 coloured by chain, front view.
PDB entry 4k44 coloured by chain, side view.
PDB entry 4k44 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Autoinhibition and phosphorylation-induced activation of phospholipase C-γ isozymes.
Hajicek N, Charpentier TH, Rush JR, Harden TK, Sondek J
Biochemistry 52 4810-9 (2013)
PMID: 23777354

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.44 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10686 (Residues: 664-766; Coverage: 8%)
Gene name: Plcg1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 48.106Å b: 53.337Å c: 150.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Dysfunction of phospholipase Cγ in immune disorders and cancer.
Koss et al. (2014)
4 more

3 mentions without citation

Autoinhibition and phosphorylation-induced activation of phospholipase C-γ isozymes.
Hajicek et al. (2013)
2 more