› 4k55

X-ray diffraction
1.91Å resolution

Structure of the extracellular domain of butyrophilin BTN3A1 in complex with (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP)

Released:
DOI: 10.2210/pdb4k55/pdb
PDB entry 4k55 coloured by chain, front view.
PDB entry 4k55 coloured by chain, side view.
PDB entry 4k55 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Butyrophilin 3A1 binds phosphorylated antigens and stimulates human γδ T cells.
Vavassori S, Kumar A, Wan GS, Ramanjaneyulu GS, Cavallari M, El Daker S, Beddoe T, Theodossis A, Williams NK, Gostick E, Price DA, Soudamini DU, Voon KK, Olivo M, Rossjohn J, Mori L, De Libero G
Nat Immunol 14 908-16 (2013)
PMID: 23872678

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Butyrophilin subfamily 3 member A1 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00481 (Residues: 28-143; Coverage: 24%)
Gene names: BTF5, BTN3A1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand H6P 1 x H6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 35.766Å b: 107.744Å c: 32.716Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.256
Expression system: Escherichia coli

Quick links

Citations

109 review citations

The burgeoning family of unconventional T cells.
Godfrey et al. (2015)
108 more

3 mentions without citation

The intracellular B30.2 domain of butyrophilin 3A1 binds phosphoantigens to mediate activation of human Vγ9Vδ2 T cells.
Sandstrom et al. (2014)
2 more