› 4k6j

X-ray diffraction
2.62Å resolution

Human cohesin inhibitor WapL

Released:
DOI: 10.2210/pdb4k6j/pdb
PDB entry 4k6j coloured by chain, front view.
PDB entry 4k6j coloured by chain, side view.
PDB entry 4k6j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human cohesin inhibitor Wapl.
Ouyang Z, Zheng G, Song J, Borek DM, Otwinowski Z, Brautigam CA, Tomchick DR, Rankin S, Yu H
Proc Natl Acad Sci U S A 110 11355-60 (2013)
PMID: 23776203

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Wings apart-like protein homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 568 amino acids
Theoretical weight: 63.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Z5K2 (Residues: 631-1190; Coverage: 47%)
Gene names: FOE, KIAA0261, WAPAL, WAPL
Sequence domains: Wings apart-like protein regulation of heterochromatin
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 107.54Å b: 107.54Å c: 300.608Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Cohesin and its regulation: on the logic of X-shaped chromosomes.
Haarhuis et al. (2014)
7 more

6 mentions without citation

Structural Insights into Ring Formation of Cohesin and Related Smc Complexes.
Gligoris et al. (2016)
5 more