› 4k71

X-ray diffraction
2.4Å resolution

Crystal structure of a high affinity Human Serum Albumin variant bound to the Neonatal Fc Receptor

Released:
DOI: 10.2210/pdb4k71/pdb
PDB entry 4k71 coloured by chain, front view.
PDB entry 4k71 coloured by chain, side view.
PDB entry 4k71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an HSA/FcRn complex reveals recycling by competitive mimicry of HSA ligands at a pH-dependent hydrophobic interface.
Schmidt MM, Townson SA, Andreucci AJ, King BM, Schirmer EB, Murillo AJ, Dombrowski C, Tisdale AW, Lowden PA, Masci AL, Kovalchin JT, Erbe DV, Wittrup KD, Furfine ES, Barnes TM
Structure 21 1966-78 (2013)
PMID: 24120761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dodecamer
PDBe Complex ID:
PDB-CPX-136177 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Albumin Chains: A, D
Molecule details ›
Chains: A, D
Length: 585 amino acids
Theoretical weight: 66.47 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P02768 (Residues: 25-609; Coverage: 99%)
Gene names: ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341
Sequence domains: Serum albumin family
Structure domains: Serum Albumin; Chain A, Domain 1
IgG receptor FcRn large subunit p51 Chains: B, E
Molecule details ›
Chains: B, E
Length: 274 amino acids
Theoretical weight: 30.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P55899 (Residues: 24-297; Coverage: 80%)
Gene names: FCGRT, FCRN
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: C, F
Molecule details ›
Chains: C, F
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 19 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21212
Unit cell:
a: 127.893Å b: 203.536Å c: 100.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.255
Expression systems:
  • Saccharomyces cerevisiae
  • Homo sapiens

Quick links

Citations

19 review citations

Albumin-based drug delivery: harnessing nature to cure disease.
Larsen et al. (2016)
18 more

9 mentions without citation

Extending serum half-life of albumin by engineering neonatal Fc receptor (FcRn) binding.
Andersen et al. (2014)
8 more