› 4k84

X-ray diffraction
1.9Å resolution

Crystal structure of human ceramide-1-phosphate transfer protein (CPTP) in complex with 16:0 ceramide-1-phosphate (16:0-C1P)

Released:
DOI: 10.2210/pdb4k84/pdb
PDB entry 4k84 coloured by chain, front view.
PDB entry 4k84 coloured by chain, side view.
PDB entry 4k84 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Non-vesicular trafficking by a ceramide-1-phosphate transfer protein regulates eicosanoids.
Simanshu DK, Kamlekar RK, Wijesinghe DS, Zou X, Zhai X, Mishra SK, Molotkovsky JG, Malinina L, Hinchcliffe EH, Chalfant CE, Brown RE, Patel DJ
Nature 500 463-7 (2013)
PMID: 23863933

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide-1-phosphate transfer protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TA50 (Residues: 1-214; Coverage: 100%)
Gene names: CPTP, GLTPD1
Sequence domains: Glycolipid transfer protein (GLTP)
Structure domains: Glycolipid transfer protein

Ligands and Environments

1 bound ligand:
Ligand 1PX 2 x 1PX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 62.604Å b: 50.291Å c: 62.87Å
α: 90° β: 100.11° γ: 90°
R-values:
R R work R free
0.165 0.162 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

43 review citations

Sphingolipids and their metabolism in physiology and disease.
Hannun et al. (2018)
42 more

7 mentions without citation

Emerging roles for human glycolipid transfer protein superfamily members in the regulation of autophagy, inflammation, and cell death.
Mishra et al. (2020)
6 more