› 4k92

X-ray diffraction
2Å resolution

A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation

Released:
DOI: 10.2210/pdb4k92/pdb
PDB entry 4k92 coloured by chain, front view.
PDB entry 4k92 coloured by chain, side view.
PDB entry 4k92 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A cryptic TOG domain with a distinct architecture underlies CLASP-dependent bipolar spindle formation.
Leano JB, Rogers SL, Slep KC
Structure 21 939-50 (2013)
PMID: 23727231

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CLIP-associating protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 30.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Z460 (Residues: 284-552; Coverage: 18%)
Gene names: CLASP1, KIAA0622, MAST1
Sequence domains: CLASP N terminal
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 62.284Å b: 66.492Å c: 138.934Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Microtubule plus-end tracking proteins in neuronal development.
van de Willige et al. (2016)
7 more

8 mentions without citation

The contribution of αβ-tubulin curvature to microtubule dynamics.
Brouhard et al. (2014)
7 more