› 4kbl

X-ray diffraction
3.3Å resolution

Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism

Released:
DOI: 10.2210/pdb4kbl/pdb
PDB entry 4kbl coloured by chain, front view.
PDB entry 4kbl coloured by chain, side view.
PDB entry 4kbl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism.
Duda DM, Olszewski JL, Schuermann JP, Kurinov I, Miller DJ, Nourse A, Alpi AF, Schulman BA
Structure 21 1030-41 (2013)
PMID: 23707686

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase ARIH1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 64.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y4X5 (Residues: 1-557; Coverage: 100%)
Gene names: ARI, ARIH1, HUSSY-27, MOP6, UBCH7BP
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41
Unit cell:
a: 147.403Å b: 147.403Å c: 86.821Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.231 0.283
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Ubiquitin modifications.
Swatek et al. (2016)
26 more

21 mentions without citation

Structural insights into the catalysis and regulation of E3 ubiquitin ligases.
Buetow et al. (2016)
20 more