› 4kc9

X-ray diffraction
3.6Å resolution

Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism

Released:
DOI: 10.2210/pdb4kc9/pdb
PDB entry 4kc9 coloured by chain, front view.
PDB entry 4kc9 coloured by chain, side view.
PDB entry 4kc9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism.
Duda DM, Olszewski JL, Schuermann JP, Kurinov I, Miller DJ, Nourse A, Alpi AF, Schulman BA
Structure 21 1030-41 (2013)
PMID: 23707686

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase ARIH1 Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 65.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y4X5 (Residues: 1-557; Coverage: 100%)
Gene names: ARI, ARIH1, HUSSY-27, MOP6, UBCH7BP
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P63
Unit cell:
a: 96.126Å b: 96.126Å c: 151.329Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.27 0.302
Expression system: Escherichia coli

Quick links

Citations

33 review citations

New insights into ubiquitin E3 ligase mechanism.
Berndsen et al. (2014)
32 more

6 mentions without citation

Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism.
Duda et al. (2013)
5 more