› 4kfm

X-ray diffraction
3.45Å resolution

Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with the beta-gamma G protein subunits

Released:
DOI: 10.2210/pdb4kfm/pdb
PDB entry 4kfm coloured by chain, front view.
PDB entry 4kfm coloured by chain, side view.
PDB entry 4kfm coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structure of the mammalian GIRK2-βγ G-protein complex.
Whorton MR, MacKinnon R
Nature 498 190-7 (2013)
PMID: 23739333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-155752 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
G protein-activated inward rectifier potassium channel 2 Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 39.06 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P48542 (Residues: 52-380; Coverage: 77%)
Gene names: Girk2, Kcnj6, Kcnj7, W
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 70 amino acids
Theoretical weight: 7.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

4 bound ligands:
Ligand PIO 1 x PIO
Ligand K 5 x K
Ligand NA 1 x NA
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I422
Unit cell:
a: 127.308Å b: 127.308Å c: 309.392Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.265
Expression systems:
  • Komagataella pastoris
  • Spodoptera frugiperda

Quick links

Citations

41 review citations

The role of D2-autoreceptors in regulating dopamine neuron activity and transmission.
Ford CP. (2014)
40 more

32 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
31 more