› 4kgh

X-ray diffraction
2.81Å resolution

Crystal Structure of human splunc1 lacking the secretion signal sequence

Released:
DOI: 10.2210/pdb4kgh/pdb
PDB entry 4kgh coloured by chain, front view.
PDB entry 4kgh coloured by chain, side view.
PDB entry 4kgh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airways.
Garland AL, Walton WG, Coakley RD, Tan CD, Gilmore RC, Hobbs CA, Tripathy A, Clunes LA, Bencharit S, Stutts MJ, Betts L, Redinbo MR, Tarran R
Proc Natl Acad Sci U S A 110 15973-8 (2013)
PMID: 24043776

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BPI fold-containing family A member 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 24.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NP55 (Residues: 19-256; Coverage: 100%)
Gene names: BPIFA1, LUNX, NASG, PLUNC, SPLUNC1, SPURT, UNQ787/PRO1606
Sequence domains: LBP / BPI / CETP family, N-terminal domain
Structure domains: Bactericidal permeability-increasing protein; domain 1

Ligands and Environments

4 bound ligands:
Ligand NO3 1 x NO3
Ligand NH4 1 x NH4
Ligand NA 1 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 47.475Å b: 203.727Å c: 118.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.246 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Origins of cystic fibrosis lung disease.
Stoltz et al. (2015)
39 more

6 mentions without citation

Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airways.
Garland et al. (2013)
5 more