4ki1

X-ray diffraction
3.2Å resolution

Primitive triclinic crystal form of the human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23

Released:
Source organism: Homo sapiens
Primary publication:
A range of Cℇ3-Cℇ4 interdomain angles in IgE Fc accommodate binding to its receptor CD23.
Acta Crystallogr F Struct Biol Commun 70 305-9 (2014)
PMID: 24598915

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-134793 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin heavy constant epsilon Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 24.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01854 (Residues: 209-427; Coverage: 40%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin epsilon Fc receptor soluble form Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 143 amino acids
Theoretical weight: 16.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06734 (Residues: 156-298; Coverage: 45%)
Gene names: CD23A, CLEC4J, FCE2, FCER2, IGEBF
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 48.79Å b: 63.84Å c: 163.89Å
α: 100.67° β: 90.13° γ: 103.49°
R-values:
R R work R free
0.234 0.232 0.266
Expression systems:
  • Homo sapiens
  • Escherichia coli