4kk8

X-ray diffraction
2.86Å resolution

Structure of the E148Q mutant of CLC-ec1 deltaNC construct in 100mM fluoride

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
hetero dimer
PDBe Complex ID:
PDB-CPX-153303 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 47.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 17-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab, heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-104; Coverage: 97%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 231.226Å b: 98.903Å c: 170.022Å
α: 90° β: 131.77° γ: 90°
R-values:
R R work R free
0.212 0.21 0.256
Expression systems:
  • Escherichia coli
  • Mus musculus