› 4km5

X-ray diffraction
2.5Å resolution

X-ray crystal structure of human cyclic GMP-AMP synthase (cGAS)

Released:
DOI: 10.2210/pdb4km5/pdb
PDB entry 4km5 coloured by chain, front view.
PDB entry 4km5 coloured by chain, side view.
PDB entry 4km5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human cGAS reveals a conserved family of second-messenger enzymes in innate immunity.
Kranzusch PJ, Lee AS, Berger JM, Doudna JA
Cell Rep 3 1362-8 (2013)
PMID: 23707061

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 157-522; Coverage: 70%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2, ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 46.454Å b: 162.7Å c: 58.407Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.24
Expression system: Escherichia coli

Quick links

Citations

85 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
84 more

14 mentions without citation

Therapeutic Development by Targeting the cGAS-STING Pathway in Autoimmune Disease and Cancer.
Li et al. (2021)
13 more