› 4kre

X-ray diffraction
1.75Å resolution

Structure of Human Argonaute-1 bound to endogenous Sf9 RNA

Released:
DOI: 10.2210/pdb4kre/pdb
PDB entry 4kre coloured by chain, front view.
PDB entry 4kre coloured by chain, side view.
PDB entry 4kre coloured by chain, top view.
Source organisms:
Primary publication:
The making of a slicer: activation of human Argonaute-1.
Faehnle CR, Elkayam E, Haase AD, Hannon GJ, Joshua-Tor L
Cell Rep 3 1901-9 (2013)
PMID: 23746446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193901 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-1 Chain: A
Molecule details ›
Chain: A
Length: 858 amino acids
Theoretical weight: 97.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UL18 (Residues: 1-857; Coverage: 100%)
Gene names: AGO1, EIF2C1
Sequence domains:
Structure domains:
RNA (5'-R(P*AP*AP*UP*AP*UP*UP*AP*AP*A*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: R
Molecule details ›
Chain: R
Length: 20 nucleotides
Theoretical weight: 6.47 KDa
Source organism: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 70.47Å b: 98.63Å c: 73.21Å
α: 90° β: 110.96° γ: 90°
R-values:
R R work R free
0.172 0.17 0.202
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

The evolutionary journey of Argonaute proteins.
Swarts et al. (2014)
19 more

9 mentions without citation

Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?
Nakanishi K. (2016)
8 more