› 4krf

X-ray diffraction
2.1Å resolution

Structure of Human Argonaute-1 let-7 complex

Released:
DOI: 10.2210/pdb4krf/pdb
PDB entry 4krf coloured by chain, front view.
PDB entry 4krf coloured by chain, side view.
PDB entry 4krf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The making of a slicer: activation of human Argonaute-1.
Faehnle CR, Elkayam E, Haase AD, Hannon GJ, Joshua-Tor L
Cell Rep 3 1901-9 (2013)
PMID: 23746446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193902 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-1 Chain: A
Molecule details ›
Chain: A
Length: 858 amino acids
Theoretical weight: 97.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UL18 (Residues: 1-857; Coverage: 100%)
Gene names: AGO1, EIF2C1
Sequence domains:
Structure domains:
RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*UP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 22 nucleotides
Theoretical weight: 7.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 70.39Å b: 97.73Å c: 72.7Å
α: 90° β: 110.89° γ: 90°
R-values:
R R work R free
0.177 0.176 0.217
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

22 review citations

The evolutionary journey of Argonaute proteins.
Swarts et al. (2014)
21 more

7 mentions without citation

Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?
Nakanishi K. (2016)
6 more