4kt1

X-ray diffraction
2.5Å resolution

Complex of R-spondin 1 with LGR4 extracellular domain

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for R-spondin recognition by LGR4/5/6 receptors.
Genes Dev 27 1339-44 (2013)
PMID: 23756652

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-173734 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Leucine-rich repeat-containing G-protein coupled receptor 4 Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 55.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXB1 (Residues: 26-527; Coverage: 54%)
Gene names: GPR48, LGR4
Sequence domains:
Structure domains: Ribonuclease Inhibitor
R-spondin-1 Chain: E
Molecule details ›
Chain: E
Length: 90 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q2MKA7 (Residues: 39-128; Coverage: 37%)
Gene name: RSPO1
Sequence domains: Furin-like repeat, cysteine-rich
Structure domains: Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A, domain 2

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P32
Unit cell:
a: 91.376Å b: 91.376Å c: 87.273Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.16 0.209
Expression system: Spodoptera frugiperda