› 4ku7

X-ray diffraction
1.65Å resolution

Structures of PKGI Reveal a cGMP-Selective Activation Mechanism

Released:
DOI: 10.2210/pdb4ku7/pdb
PDB entry 4ku7 coloured by chain, front view.
PDB entry 4ku7 coloured by chain, side view.
PDB entry 4ku7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for cyclic-nucleotide selectivity and cGMP-selective activation of PKG I.
Huang GY, Kim JJ, Reger AS, Lorenz R, Moon EW, Zhao C, Casteel DE, Bertinetti D, Vanschouwen B, Selvaratnam R, Pflugrath JW, Sankaran B, Melacini G, Herberg FW, Kim C
Structure 22 116-24 (2014)
PMID: 24239458

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13976 (Residues: 204-354; Coverage: 23%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand IOD 24 x IOD
Ligand PCG 1 x PCG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P41212
Unit cell:
a: 47.118Å b: 47.118Å c: 101.799Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Allosteric inhibition explained through conformational ensembles sampling distinct "mixed" states.
Byun et al. (2020)
5 more

11 mentions without citation

Cyclic nucleotide selectivity of protein kinase G isozymes.
Kim et al. (2021)
10 more