› 4kvg

X-ray diffraction
1.65Å resolution

Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1

Released:
DOI: 10.2210/pdb4kvg/pdb
PDB entry 4kvg coloured by chain, front view.
PDB entry 4kvg coloured by chain, side view.
PDB entry 4kvg coloured by chain, top view.
Source organisms:
Primary publication:
The structure of Rap1 in complex with RIAM reveals specificity determinants and recruitment mechanism.
Zhang H, Chang YC, Brennan ML, Wu J
J Mol Cell Biol 6 128-39 (2014)
PMID: 24287201

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158729 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rap-1A Chains: A, C
Molecule details ›
Chains: A, C
Length: 168 amino acids
Theoretical weight: 19.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62834 (Residues: 1-167; Coverage: 91%)
Gene names: KREV1, RAP1A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 260 amino acids
Theoretical weight: 30.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R5A3 (Residues: 179-437; Coverage: 39%)
Gene names: Apbb1ip, Prel1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 82.467Å b: 85.848Å c: 160.852Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.219
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structures of Ras superfamily effector complexes: What have we learnt in two decades?
Mott et al. (2015)
5 more

6 mentions without citation

Volatile anesthetics affect macrophage phagocytosis.
Zha et al. (2019)
5 more