› 4l01

X-ray diffraction
1.9Å resolution

Crystal structure of the V658F apo Jak1 pseudokinase domain

Released:
DOI: 10.2210/pdb4l01/pdb
PDB entry 4l01 coloured by chain, front view.
PDB entry 4l01 coloured by chain, side view.
PDB entry 4l01 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a pseudokinase-domain switch that controls oncogenic activation of Jak kinases.
Toms AV, Deshpande A, McNally R, Jeong Y, Rogers JM, Kim CU, Gruner SM, Ficarro SB, Marto JA, Sattler M, Griffin JD, Eck MJ
Nat Struct Mol Biol 20 1221-3 (2013)
PMID: 24013208

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149968 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P23458 (Residues: 561-860; Coverage: 26%)
Gene names: JAK1, JAK1A, JAK1B
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 47.288Å b: 80.557Å c: 76.555Å
α: 90° β: 95.29° γ: 90°
R-values:
R R work R free
0.204 0.202 0.233
Expression system: Not provided

Quick links

Citations

18 review citations

Mechanisms and consequences of Jak-STAT signaling in the immune system.
Villarino et al. (2017)
17 more

4 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
3 more