› 4l0z

X-ray diffraction
2.7Å resolution

Crystal structure of Runx1 and Ets1 bound to TCR alpha promoter (crystal form 2)

Released:
DOI: 10.2210/pdb4l0z/pdb
PDB entry 4l0z coloured by chain, front view.
PDB entry 4l0z coloured by chain, side view.
PDB entry 4l0z coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Ets1 activation by Runx1.
Shrivastava T, Mino K, Babayeva ND, Baranovskaya OI, Rizzino A, Tahirov TH
Leukemia 28 2040-8 (2014)
PMID: 24646888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117769 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Runt-related transcription factor 1 Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 26.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03347 (Residues: 1-242; Coverage: 54%)
Gene names: Aml1, Cbfa2, Pebp2ab, Runx1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like
Protein C-ets-1 Chain: B
Molecule details ›
Chain: B
Length: 146 amino acids
Theoretical weight: 16.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14921 (Residues: 296-441; Coverage: 33%)
Gene names: ETS1, EWSR2
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*GP*AP*AP*GP*CP*CP*AP*CP*AP*TP*CP*CP*TP*CP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
5'-D(*CP*AP*GP*AP*GP*GP*AP*TP*GP*TP*GP*GP*CP*TP*TP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.95 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 87.343Å b: 100.14Å c: 67.225Å
α: 90° β: 120.49° γ: 90°
R-values:
R R work R free
0.22 0.22 0.272
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting transcription factors in cancer - from undruggable to reality.
Bushweller JH. (2019)
2 more

4 mentions without citation

ETS transcription factors induce a unique UV damage signature that drives recurrent mutagenesis in melanoma.
Mao et al. (2018)
3 more