› 4l18

X-ray diffraction
2.3Å resolution

Crystal structure of Runx1 and Ets1 bound to TCR alpha promoter (crystal form 3)

Released:
DOI: 10.2210/pdb4l18/pdb
PDB entry 4l18 coloured by chain, front view.
PDB entry 4l18 coloured by chain, side view.
PDB entry 4l18 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Ets1 activation by Runx1.
Shrivastava T, Mino K, Babayeva ND, Baranovskaya OI, Rizzino A, Tahirov TH
Leukemia 28 2040-8 (2014)
PMID: 24646888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117770 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Runt-related transcription factor 1 Chains: A, E
Molecule details ›
Chains: A, E
Length: 167 amino acids
Theoretical weight: 18.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03347 (Residues: 48-214; Coverage: 37%)
Gene names: Aml1, Cbfa2, Pebp2ab, Runx1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like
Protein C-ets-1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 146 amino acids
Theoretical weight: 16.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14921 (Residues: 296-441; Coverage: 33%)
Gene names: ETS1, EWSR2
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*GP*AP*AP*GP*CP*CP*AP*CP*AP*TP*CP*CP*TP*CP*T)-3' Chains: C, G
Molecule details ›
Chains: C, G
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
5'-D(*CP*AP*GP*AP*GP*GP*AP*TP*GP*TP*GP*GP*CP*TP*TP*C)-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 16 nucleotides
Theoretical weight: 4.95 KDa

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 66.153Å b: 100.032Å c: 72.064Å
α: 90° β: 110.23° γ: 90°
R-values:
R R work R free
0.231 0.231 0.28
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting transcription factors in cancer - from undruggable to reality.
Bushweller JH. (2019)
2 more

2 mentions without citation

ETS transcription factors induce a unique UV damage signature that drives recurrent mutagenesis in melanoma.
Mao et al. (2018)
1 more