› 4l2w

X-ray diffraction
2.49Å resolution

Crystal structure of the Shroom-Binding domain of human Rock1

Released:
DOI: 10.2210/pdb4l2w/pdb
PDB entry 4l2w coloured by chain, front view.
PDB entry 4l2w coloured by chain, side view.
PDB entry 4l2w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a highly conserved domain of Rock1 required for Shroom-mediated regulation of cell morphology.
Mohan S, Das D, Bauer RJ, Heroux A, Zalewski JK, Heber S, Dosunmu-Ogunbi AM, Trakselis MA, Hildebrand JD, Vandemark AP
PLoS One 8 e81075 (2013)
PMID: 24349032

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-associated protein kinase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 87 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13464 (Residues: 834-914; Coverage: 6%)
Gene name: ROCK1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 142.5Å b: 56.23Å c: 80.74Å
α: 90° β: 119.14° γ: 90°
R-values:
R R work R free
0.238 0.236 0.276
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Coiled-coils: The long and short of it.
Truebestein et al. (2016)
2 more

2 mentions without citation

Structure of a highly conserved domain of Rock1 required for Shroom-mediated regulation of cell morphology.
Mohan et al. (2013)
1 more