› 4l3i

X-ray diffraction
3.6Å resolution

Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)

Released:
DOI: 10.2210/pdb4l3i/pdb
PDB entry 4l3i coloured by chain, front view.
PDB entry 4l3i coloured by chain, side view.
PDB entry 4l3i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Marking and measuring single microtubules by PRC1 and kinesin-4.
Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM
Cell 154 377-90 (2013)
PMID: 23870126

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein regulator of cytokinesis 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 58.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O43663 (Residues: 1-486; Coverage: 78%)
Gene name: PRC1
Sequence domains: Microtubule associated protein (MAP65/ASE1 family)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 61.205Å b: 210.782Å c: 112.294Å
α: 90° β: 105.81° γ: 90°
R-values:
R R work R free
0.262 0.257 0.3
Expression system: Escherichia coli BL21

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Citations

15 review citations

The contribution of αβ-tubulin curvature to microtubule dynamics.
Brouhard et al. (2014)
14 more