› 4l4s

X-ray diffraction
2.9Å resolution

Structural characterisation of the NADH binary complex of human lactate dehydrogenase M isozyme

Released:
DOI: 10.2210/pdb4l4s/pdb
PDB entry 4l4s coloured by chain, front view.
PDB entry 4l4s coloured by chain, side view.
PDB entry 4l4s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of the apo form and NADH binary complex of human lactate dehydrogenase.
Dempster S, Harper S, Moses JE, Dreveny I
Acta Crystallogr D Biol Crystallogr 70 1484-90 (2014)
PMID: 24816116

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 339 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P4122
Unit cell:
a: 84.317Å b: 84.317Å c: 276.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.246
Expression system: Escherichia coli

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Citations

4 review citations

Liver glucose metabolism in humans.
Adeva-Andany et al. (2016)
3 more

7 mentions without citation

Small Molecule-Based Enzyme Inhibitors in the Treatment of Primary Hyperoxalurias.
Moya-Garzon et al. (2021)
6 more