› 4l9m

X-ray diffraction
3Å resolution

Autoinhibited state of the Ras-specific exchange factor RasGRP1

Released:
DOI: 10.2210/pdb4l9m/pdb
PDB entry 4l9m coloured by chain, front view.
PDB entry 4l9m coloured by chain, side view.
PDB entry 4l9m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1.
Iwig JS, Vercoulen Y, Das R, Barros T, Limnander A, Che Y, Pelton JG, Wemmer DE, Roose JP, Kuriyan J
Elife 2 e00813 (2013)
PMID: 23908768

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAS guanyl-releasing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 64.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95267 (Residues: 50-607; Coverage: 70%)
Gene names: RASGRP, RASGRP1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CIT 1 x CIT
Ligand GOL 1 x GOL
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6122
Unit cell:
a: 76.38Å b: 76.38Å c: 408.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.224 0.269
Expression system: Escherichia coli

Quick links

Citations

8 review citations

RAP1-GTPase signaling and platelet function.
Stefanini et al. (2016)
7 more

7 mentions without citation

A Histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.
Vercoulen et al. (2017)
6 more