› 4l9u

X-ray diffraction
1.6Å resolution

Structure of C-terminal coiled coil of RasGRP1

Released:
DOI: 10.2210/pdb4l9u/pdb
PDB entry 4l9u coloured by chain, front view.
PDB entry 4l9u coloured by chain, side view.
PDB entry 4l9u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1.
Iwig JS, Vercoulen Y, Das R, Barros T, Limnander A, Che Y, Pelton JG, Wemmer DE, Roose JP, Kuriyan J
Elife 2 e00813 (2013)
PMID: 23908768

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAS guanyl-releasing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 56 amino acids
Theoretical weight: 6.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95267 (Residues: 739-793; Coverage: 7%)
Gene names: RASGRP, RASGRP1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 24.878Å b: 165.047Å c: 28.319Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.235
Expression system: Escherichia coli

Quick links

Citations

8 review citations

RAP1-GTPase signaling and platelet function.
Stefanini et al. (2016)
7 more

2 mentions without citation

Modeling of RAS complexes supports roles in cancer for less studied partners.
Engin et al. (2017)
1 more