› 4lbl

X-ray diffraction
1.58Å resolution

Crystal structure of Human galectin-3 CRD K176L mutant in complex with a-GM3

Released:
DOI: 10.2210/pdb4lbl/pdb
PDB entry 4lbl coloured by chain, front view.
PDB entry 4lbl coloured by chain, side view.
PDB entry 4lbl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Galectin-3 interactions with glycosphingolipids.
Collins PM, Bum-Erdene K, Yu X, Blanchard H
J Mol Biol 426 1439-51 (2014)
PMID: 24326249

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-3 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17931 (Residues: 114-250; Coverage: 55%)
Gene names: LGALS3, MAC2
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 35.86Å b: 57.61Å c: 62.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Galectin-3: One Molecule for an Alphabet of Diseases, from A to Z.
Sciacchitano et al. (2018)
9 more