› 4lcj

X-ray diffraction
2.86Å resolution

CtBP2 in complex with substrate MTOB

Released:
DOI: 10.2210/pdb4lcj/pdb
PDB entry 4lcj coloured by chain, front view.
PDB entry 4lcj coloured by chain, side view.
PDB entry 4lcj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human CtBP in complex with substrate MTOB reveal active site features useful for inhibitor design.
Hilbert BJ, Grossman SR, Schiffer CA, Royer WE
FEBS Lett 588 1743-8 (2014)
PMID: 24657618

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-terminal-binding protein 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 349 amino acids
Theoretical weight: 38.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56545 (Residues: 31-362; Coverage: 75%)
Gene name: CTBP2
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAD 8 x NAD
Ligand KMT 8 x KMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 86.151Å b: 140.605Å c: 135.126Å
α: 90° β: 97.87° γ: 90°
R-values:
R R work R free
0.216 0.214 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

CtBP- an emerging oncogene and novel small molecule drug target: Advances in the understanding of its oncogenic action and identification of therapeutic inhibitors.
Dcona et al. (2017)
2 more

6 mentions without citation

Assembly of human C-terminal binding protein (CtBP) into tetramers.
Bellesis et al. (2018)
5 more