› 4lde

X-ray diffraction
2.79Å resolution

Structure of beta2 adrenoceptor bound to BI167107 and an engineered nanobody

Released:
DOI: 10.2210/pdb4lde/pdb
PDB entry 4lde coloured by chain, front view.
PDB entry 4lde coloured by chain, side view.
PDB entry 4lde coloured by chain, top view.
Source organisms:
Primary publication:
Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody.
Ring AM, Manglik A, Kruse AC, Enos MD, Weis WI, Garcia KC, Kobilka BK
Nature 502 575-579 (2013)
PMID: 24056936

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208290 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 53.47 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: P07550 (Residues: 29-348; Coverage: 71%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
Structure domains:
Camelid Antibody Fragment Chain: B
Molecule details ›
Chain: B
Length: 120 amino acids
Theoretical weight: 12.95 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand P0G 1 x P0G
Ligand NA 1 x NA
Ligand 1WV 1 x 1WV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 49.67Å b: 66.48Å c: 303.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.256
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

51 review citations

The Molecular Basis of G Protein-Coupled Receptor Activation.
Weis et al. (2018)
50 more

69 mentions without citation

Nanobodies to Study G Protein-Coupled Receptor Structure and Function.
Manglik et al. (2017)
68 more