› 4le9

X-ray diffraction
1.34Å resolution

Crystal structure of a chimeric c-Src-SH3 domain

Released:
DOI: 10.2210/pdb4le9/pdb
PDB entry 4le9 coloured by chain, front view.
PDB entry 4le9 coloured by chain, side view.
PDB entry 4le9 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
3D domain swapping in a chimeric c-Src SH3 domain takes place through two hinge loops.
Cámara-Artigas A, Martínez-Rodríguez S, Ortiz-Salmerón E, Martín-García JM
J Struct Biol 186 195-203 (2014)
PMID: 24556574

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.68 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00523 (Residues: 85-141; Coverage: 11%)
Gene name: SRC
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6322
Unit cell:
a: 68.239Å b: 68.239Å c: 46.495Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.137 0.136 0.164
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Crystallographic studies on protein misfolding: Domain swapping and amyloid formation in the SH3 domain.
Cámara-Artigas A. (2016)
1 more