› 4lew

X-ray diffraction
2.04Å resolution

Structure of human cGAS

Released:
DOI: 10.2210/pdb4lew/pdb
PDB entry 4lew coloured by chain, front view.
PDB entry 4lew coloured by chain, side view.
PDB entry 4lew coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cyclic GMP-AMP synthase is activated by double-stranded DNA-induced oligomerization.
Li X, Shu C, Yi G, Chaton CT, Shelton CL, Diao J, Zuo X, Kao CC, Herr AB, Li P
Immunity 39 1019-31 (2013)
PMID: 24332030

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 42.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 157-522; Coverage: 70%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 46.815Å b: 111.48Å c: 76.414Å
α: 90° β: 92.73° γ: 90°
R-values:
R R work R free
0.18 0.179 0.223
Expression system: Escherichia coli

Quick links

Citations

130 review citations

Innate immune sensing and signaling of cytosolic nucleic acids.
Wu et al. (2014)
129 more

3 mentions without citation

Cyclic GMP-AMP synthase is activated by double-stranded DNA-induced oligomerization.
Li et al. (2013)
2 more