› 4lg4

X-ray diffraction
2.42Å resolution

Structural Basis for Autoactivation of Human Mst2 Kinase and Its Regulation by RASSF5

Released:
DOI: 10.2210/pdb4lg4/pdb
PDB entry 4lg4 coloured by chain, front view.
PDB entry 4lg4 coloured by chain, side view.
PDB entry 4lg4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5.
Ni L, Li S, Yu J, Min J, Brautigam CA, Tomchick DR, Pan D, Luo X
Structure 21 1757-68 (2013)
PMID: 23972470

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase 3 36kDa subunit Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 299 amino acids
Theoretical weight: 33.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13188 (Residues: 16-313; Coverage: 61%)
Gene names: KRS1, MST2, STK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 227.179Å b: 69.771Å c: 148.082Å
α: 90° β: 108.61° γ: 90°
R-values:
R R work R free
0.194 0.193 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

19 review citations

Mechanisms of Hippo pathway regulation.
Meng et al. (2016)
18 more

8 mentions without citation

Activation mechanisms of the Hippo kinase signaling cascade.
Bae et al. (2018)
7 more