› 4lly

X-ray diffraction
1.6Å resolution

Crystal structure of Pertuzumab Clambda Fab with variable and constant domain redesigns (VRD2 and CRD2) at 1.6A

Released:
DOI: 10.2210/pdb4lly/pdb
PDB entry 4lly coloured by chain, front view.
PDB entry 4lly coloured by chain, side view.
PDB entry 4lly coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface.
Lewis SM, Wu X, Pustilnik A, Sereno A, Huang F, Rick HL, Guntas G, Leaver-Fay A, Smith EM, Ho C, Hansen-Estruch C, Chamberlain AK, Truhlar SM, Conner EM, Atwell S, Kuhlman B, Demarest SJ
Nat Biotechnol 32 191-8 (2014)
PMID: 24463572

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143707 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 226 amino acids
Theoretical weight: 23.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: S6B291 (Residues: 20-71, 72-117, 118-243; Coverage: 50%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin lambda constant 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 212 amino acids
Theoretical weight: 23.17 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOY2 (Residues: 1-105; Coverage: 99%)
Gene name: IGLC2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 52.772Å b: 52.847Å c: 89.483Å
α: 76.88° β: 84.32° γ: 85.53°
R-values:
R R work R free
0.199 0.198 0.229
Expression system: Homo sapiens

Quick links

Citations

47 review citations

Alternative molecular formats and therapeutic applications for bispecific antibodies.
Spiess et al. (2015)
46 more

1 mention without citation

Immunotherapy-induced neutralizing antibodies disrupt allergen binding and sustain allergen tolerance in peanut allergy.
LaHood et al. (2023)