› 4lmq

X-ray diffraction
2.77Å resolution

Development and Preclinical Characterization of a Humanized Antibody Targeting CXCL12

Released:
DOI: 10.2210/pdb4lmq/pdb
PDB entry 4lmq coloured by chain, front view.
PDB entry 4lmq coloured by chain, side view.
PDB entry 4lmq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Development and preclinical characterization of a humanized antibody targeting CXCL12.
Zhong C, Wang J, Li B, Xiang H, Ultsch M, Coons M, Wong T, Chiang NY, Clark S, Clark R, Quintana L, Gribling P, Suto E, Barck K, Corpuz R, Yao J, Takkar R, Lee WP, Damico-Beyer LA, Carano RD, Adams C, Kelley RF, Wang W, Ferrara N
Clin Cancer Res 19 4433-45 (2013)
PMID: 23812669

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211269 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SDF-1-alpha(3-67) Chains: D, F
Molecule details ›
Chains: D, F
Length: 61 amino acids
Theoretical weight: 7.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48061 (Residues: 29-89; Coverage: 85%)
Gene names: CXCL12, SDF1, SDF1A, SDF1B
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
hu30D8 Fab heavy chain Chains: E, H
Molecule details ›
Chains: E, H
Length: 221 amino acids
Theoretical weight: 23.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
hu30D8 Fab light chain Chains: I, L
Molecule details ›
Chains: I, L
Length: 214 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 64.949Å b: 73.101Å c: 106.626Å
α: 90° β: 90.4° γ: 90°
R-values:
R R work R free
0.255 0.253 0.301
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The intricate role of CXCR4 in cancer.
Chatterjee et al. (2014)
18 more