› 4lnu

X-ray diffraction
2.19Å resolution

Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin

Released:
DOI: 10.2210/pdb4lnu/pdb
PDB entry 4lnu coloured by chain, front view.
PDB entry 4lnu coloured by chain, side view.
PDB entry 4lnu coloured by chain, top view.
Source organisms:
Primary publication:
The structure of apo-kinesin bound to tubulin links the nucleotide cycle to movement.
Cao L, Wang W, Jiang Q, Wang C, Knossow M, Gigant B
Nat Commun 5 5364 (2014)
PMID: 25395082

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-112166 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QC38 (Residues: 4-454; Coverage: 99%)
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50.07 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Designed ankyrin repeat protein (DARPIN) D1 Chain: D
Molecule details ›
Chain: D
Length: 169 amino acids
Theoretical weight: 18.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Kinesin-1 heavy chain Chain: K
Molecule details ›
Chain: K
Length: 325 amino acids
Theoretical weight: 36.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P33176 (Residues: 1-325; Coverage: 34%)
Gene names: KIF5B, KNS, KNS1
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

6 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand SO4 11 x SO4
Ligand GDP 1 x GDP
Ligand MES 1 x MES
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 73.4Å b: 83.01Å c: 83.307Å
α: 116.16° β: 105.08° γ: 97.57°
R-values:
R R work R free
0.157 0.155 0.195
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies.
Knossow et al. (2020)
4 more

29 mentions without citation

Review: Mechanochemistry of the kinesin-1 ATPase.
Cross RA. (2016)
28 more