› 4log

X-ray diffraction
2.7Å resolution

The crystal structure of the orphan nuclear receptor PNR ligand binding domain fused with MBP

Released:
DOI: 10.2210/pdb4log/pdb
PDB entry 4log coloured by chain, front view.
PDB entry 4log coloured by chain, side view.
PDB entry 4log coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of the orphan nuclear receptor NR2E3/PNR ligand binding domain reveals a dimeric auto-repressed conformation.
Tan MH, Zhou XE, Soon FF, Li X, Li J, Yong EL, Melcher K, Xu HE
PLoS One 8 e74359 (2013)
PMID: 24069298

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein; Photoreceptor-specific nuclear receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 63.69 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
  • Canonical: Q9Y5X4 (Residues: 217-410; Coverage: 47%)
Gene names: JW3994, NR2E3, PNR, RNR, b4034, malE
Sequence domains:
Structure domains: Retinoid X Receptor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 89.435Å b: 184.936Å c: 85.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.285 0.307
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Advances in our structural understanding of orphan nuclear receptors.
Gallastegui et al. (2015)
3 more

9 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
8 more