› 4lqd

X-ray diffraction
2.45Å resolution

The crystal structures of the Brucella protein TcpB and the TLR adaptor protein TIRAP show structural differences in microbial TIR mimicry

Released:
DOI: 10.2210/pdb4lqd/pdb
PDB entry 4lqd coloured by chain, front view.
PDB entry 4lqd coloured by chain, side view.
PDB entry 4lqd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry.
Snyder GA, Deredge D, Waldhuber A, Fresquez T, Wilkins DZ, Smith PT, Durr S, Cirl C, Jiang J, Jennings W, Luchetti T, Snyder N, Sundberg EJ, Wintrode P, Miethke T, Xiao TS
J Biol Chem 289 669-79 (2014)
PMID: 24275656

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toll/interleukin-1 receptor domain-containing adapter protein Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58753 (Residues: 81-221; Coverage: 64%)
Gene names: MAL, TIRAP
Sequence domains: TIR domain
Structure domains: Toll/interleukin-1 receptor homology (TIR) domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 87.57Å b: 87.57Å c: 80.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.218
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Assembly and localization of Toll-like receptor signalling complexes.
Gay et al. (2014)
16 more

4 mentions without citation

Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry.
Snyder et al. (2014)
3 more