› 4lrn

X-ray diffraction
1.89Å resolution

Ontogeny of recognition specificity and functionality for the anti-HIV antibody 4E10

Released:
DOI: 10.2210/pdb4lrn/pdb
PDB entry 4lrn coloured by chain, front view.
PDB entry 4lrn coloured by chain, side view.
PDB entry 4lrn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ontogeny of recognition specificity and functionality for the broadly neutralizing anti-HIV antibody 4E10.
Finton KA, Friend D, Jaffe J, Gewe M, Holmes MA, Larman HB, Stuart A, Larimore K, Greenberg PD, Elledge SJ, Stamatatos L, Strong RK
PLoS Pathog 10 e1004403 (2014)
PMID: 25254371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208455 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GEP 1 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 127 amino acids
Theoretical weight: 13.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin kappa variable 3-20 Chain: L
Molecule details ›
Chain: L
Length: 111 amino acids
Theoretical weight: 11.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01619 (Residues: 21-116; Coverage: 100%)
Gene name: IGKV3-20
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 35.494Å b: 48.733Å c: 110.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.233
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Germline-targeting immunogens.
Stamatatos et al. (2017)
17 other articles

2 mentions without citation

Potent and protective IGHV3-53/3-66 public antibodies and their shared escape mutant on the spike of SARS-CoV-2.
Zhang et al. (2021)
1 more