› 4m3p

X-ray diffraction
1.9Å resolution

Betaine-Homocysteine S-Methyltransferase from Homo sapiens complexed with Homocysteine

Released:
DOI: 10.2210/pdb4m3p/pdb
PDB entry 4m3p coloured by chain, front view.
PDB entry 4m3p coloured by chain, side view.
PDB entry 4m3p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Specific potassium ion interactions facilitate homocysteine binding to betaine-homocysteine S-methyltransferase.
Mládková J, Hladílková J, Diamond CE, Tryon K, Yamada K, Garrow TA, Jungwirth P, Koutmos M, Jiráček J
Proteins 82 2552-64 (2014)
PMID: 24895213

Function and Biology Details

Reaction catalysed: 
Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Betaine--homocysteine S-methyltransferase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 45.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93088 (Residues: 1-406; Coverage: 100%)
Gene name: BHMT
Sequence domains: Homocysteine S-methyltransferase
Structure domains: Homocysteine-binding-like domain

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand K 4 x K
Ligand HCS 4 x HCS
Ligand SRT 2 x SRT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 86.097Å b: 102.617Å c: 96.228Å
α: 90° β: 101.76° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Homocysteine as a Predictor of Paroxysmal Atrial Fibrillation-Related Events: A Scoping Review of the Literature.
Charalampidis et al. (2022)
1 more

1 mention without citation

1H NMR-Based Metabolomics Coupled With Molecular Docking Reveal the Anti-Diabetic Effects and Potential Active Components of Berberis vernae on Type 2 Diabetic Rats.
Li et al. (2020)