› 4m5s

X-ray diffraction
1.37Å resolution

Human alphaB crystallin core domain in complex with C-terminal peptide

Released:
DOI: 10.2210/pdb4m5s/pdb
PDB entry 4m5s coloured by chain, front view.
PDB entry 4m5s coloured by chain, side view.
PDB entry 4m5s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structured core domain of αB-crystallin can prevent amyloid fibrillation and associated toxicity.
Hochberg GK, Ecroyd H, Liu C, Cox D, Cascio D, Sawaya MR, Collier MP, Stroud J, Carver JA, Baldwin AJ, Robinson CV, Eisenberg DS, Benesch JL, Laganowsky A
Proc Natl Acad Sci U S A 111 E1562-70 (2014)
PMID: 24711386

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135874 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-crystallin B chain Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02511 (Residues: 68-153; Coverage: 49%)
Gene names: CRYA2, CRYAB, HSPB5
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like
Alpha-crystallin B chain Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02511 (Residues: 156-164; Coverage: 5%)
Gene names: CRYA2, CRYAB, HSPB5

Ligands and Environments

1 bound ligand:
Ligand SIN 1 x SIN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 35.38Å b: 47.86Å c: 122.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

A first line of stress defense: small heat shock proteins and their function in protein homeostasis.
Haslbeck et al. (2015)
26 more

11 mentions without citation

Mechanisms of Small Heat Shock Proteins.
Janowska et al. (2019)
10 more