› 4m62

X-ray diffraction
1.8Å resolution

Ontogeny of recognition specificity and functionality for the anti-HIV neutralizing antibody 4E10

Released:
DOI: 10.2210/pdb4m62/pdb
PDB entry 4m62 coloured by chain, front view.
PDB entry 4m62 coloured by chain, side view.
PDB entry 4m62 coloured by chain, top view.
Source organisms:
Primary publication:
Ontogeny of recognition specificity and functionality for the broadly neutralizing anti-HIV antibody 4E10.
Finton KA, Friend D, Jaffe J, Gewe M, Holmes MA, Larman HB, Stuart A, Larimore K, Greenberg PD, Elledge SJ, Stamatatos L, Strong RK
PLoS Pathog 10 e1004403 (2014)
PMID: 25254371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208452 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa variable 3-20 Chains: L, M
Molecule details ›
Chains: L, M
Length: 113 amino acids
Theoretical weight: 12.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01619 (Residues: 21-116; Coverage: 100%)
Gene name: IGKV3-20
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
GEP2 FV heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 127 amino acids
Theoretical weight: 13.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
T117 Chains: S, T
Molecule details ›
Chains: S, T
Length: 155 amino acids
Theoretical weight: 17.62 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 69.88Å b: 103.48Å c: 109.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.216
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Germline-targeting immunogens.
Stamatatos et al. (2017)
17 other articles

5 mentions without citation

Computationally-driven identification of antibody epitopes.
Hua et al. (2017)
4 more