› 4m6r

X-ray diffraction
2Å resolution

Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme

Released:
DOI: 10.2210/pdb4m6r/pdb
PDB entry 4m6r coloured by chain, front view.
PDB entry 4m6r coloured by chain, side view.
PDB entry 4m6r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.
Kang W, Hong SH, Lee HM, Kim NY, Lim YC, Le le TM, Lim B, Kim HC, Kim TY, Ashida H, Yokota A, Hah SS, Chun KH, Jung YK, Yang JK
Proc Natl Acad Sci U S A 111 E54-61 (2014)
PMID: 24367089

Function and Biology Details

Reaction catalysed: 
S-methyl-5-thio-D-ribulose 1-phosphate = 5-(methylthio)-2,3-dioxopentyl phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylthioribulose-1-phosphate dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96GX9 (Residues: 20-242; Coverage: 92%)
Gene names: APIP, CGI-29
Sequence domains: Class II Aldolase and Adducin N-terminal domain
Structure domains: Class II aldolase/adducin N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2221
Unit cell:
a: 107.035Å b: 107.735Å c: 192.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Advances of Zinc Signaling Studies in Prostate Cancer.
Li et al. (2020)
1 more

2 mentions without citation

Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.
Kang et al. (2014)
1 more