› 4m6t

X-ray diffraction
2.5Å resolution

Structure of human Paf1 and Leo1 complex

Released:
DOI: 10.2210/pdb4m6t/pdb
PDB entry 4m6t coloured by chain, front view.
PDB entry 4m6t coloured by chain, side view.
PDB entry 4m6t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Paf1 complex assembly and histone binding.
Chu X, Qin X, Xu H, Li L, Wang Z, Li F, Xie X, Zhou H, Shen Y, Long J
Nucleic Acids Res 41 10619-29 (2013)
PMID: 24038468

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-185430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase II-associated factor 1 homolog; RNA polymerase-associated protein LEO1 Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 21.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N7H5 (Residues: 170-259; Coverage: 17%)
  • Canonical: Q8WVC0 (Residues: 370-462; Coverage: 14%)
Gene names: LEO1, PAF1, PD2, RDL
Sequence domains: Leo1-like protein

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R32
Unit cell:
a: 115.168Å b: 115.168Å c: 157.437Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.232 0.268
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Emerging Insights into the Roles of the Paf1 Complex in Gene Regulation.
Van Oss et al. (2017)
1 more

4 mentions without citation

The Paf1 Complex: A Keystone of Nuclear Regulation Operating at the Interface of Transcription and Chromatin.
Francette et al. (2021)
3 more