4m8o

X-ray diffraction
2.2Å resolution

TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117832 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase epsilon catalytic subunit A Chain: A
Molecule details ›
Chain: A
Length: 1228 amino acids
Theoretical weight: 141.48 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21951 (Residues: 1-1228; Coverage: 55%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
PRIMER DNA Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 4.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
TEMPLATE DNA Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 148.495Å b: 68.919Å c: 149.852Å
α: 90° β: 109.5° γ: 90°
R-values:
R R work R free
0.183 0.18 0.237
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided