› 4m8q

X-ray diffraction
2.89Å resolution

Ontogeny of recognition specificity and functionality for the anti-HIV neutralizing antibody 4E10

Released:
DOI: 10.2210/pdb4m8q/pdb
PDB entry 4m8q coloured by chain, front view.
PDB entry 4m8q coloured by chain, side view.
PDB entry 4m8q coloured by chain, top view.
Source organisms:
Primary publication:
Ontogeny of recognition specificity and functionality for the broadly neutralizing anti-HIV antibody 4E10.
Finton KA, Friend D, Jaffe J, Gewe M, Holmes MA, Larman HB, Stuart A, Larimore K, Greenberg PD, Elledge SJ, Stamatatos L, Strong RK
PLoS Pathog 10 e1004403 (2014)
PMID: 25254371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-208453 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GEP1 Fv heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 129 amino acids
Theoretical weight: 13.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin kappa variable 3-20 Chains: B, L
Molecule details ›
Chains: B, L
Length: 113 amino acids
Theoretical weight: 12.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01619 (Residues: 21-116; Coverage: 100%)
Gene name: IGKV3-20
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
4E10 epitope scaffold T117 Chains: C, S
Molecule details ›
Chains: C, S
Length: 161 amino acids
Theoretical weight: 18.45 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 72.561Å b: 77.674Å c: 77.823Å
α: 90° β: 112.03° γ: 90°
R-values:
R R work R free
0.263 0.261 0.302
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Germline-targeting immunogens.
Stamatatos et al. (2017)
17 other articles

2 mentions without citation

B cell genomics behind cross-neutralization of SARS-CoV-2 variants and SARS-CoV.
Scheid et al. (2021)
1 more