› 4ma4

X-ray diffraction
2.23Å resolution

S-glutathionylated PFKFB3

Released:
DOI: 10.2210/pdb4ma4/pdb
PDB entry 4ma4 coloured by chain, front view.
PDB entry 4ma4 coloured by chain, side view.
PDB entry 4ma4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PFKFB3 regulates oxidative stress homeostasis via its S-glutathionylation in cancer.
Seo M, Lee YH
J Mol Biol 426 830-42 (2014)
PMID: 24295899

Function and Biology Details

Reactions catalysed: 
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 59.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16875 (Residues: 1-520; Coverage: 100%)
Gene name: PFKFB3
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ADP 1 x ADP
Ligand MLA 2 x MLA
Ligand ACY 1 x ACY
Ligand GSH 1 x GSH
Ligand F6P 1 x F6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6522
Unit cell:
a: 102.508Å b: 102.508Å c: 260.606Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.242
Expression system: Escherichia coli

Quick links

Citations

16 review citations

S-glutathionylation reactions in mitochondrial function and disease.
Mailloux et al. (2014)
15 more

1 mention without citation

PFKFB3 regulates oxidative stress homeostasis via its S-glutathionylation in cancer.
Seo et al. (2014)