› 4mcr

X-ray diffraction
1.65Å resolution

A high resolution structure of human glutamate carboxypeptidase II (GCPII) in complex with folyltri-gamma-L-glutamic acid (pteroyltetra-gamma-L-glutamic acid)

Released:
DOI: 10.2210/pdb4mcr/pdb
PDB entry 4mcr coloured by chain, front view.
PDB entry 4mcr coloured by chain, side view.
PDB entry 4mcr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical characterization of the folyl-poly-γ-l-glutamate hydrolyzing activity of human glutamate carboxypeptidase II.
Navrátil M, Ptáček J, Šácha P, Starková J, Lubkowski J, Bařinka C, Konvalinka J
FEBS J 281 3228-42 (2014)
PMID: 24863754

Function and Biology Details

Reaction catalysed: 
Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glutamate carboxypeptidase 2 Chain: A
Molecule details ›
Chain: A
Length: 757 amino acids
Theoretical weight: 84.97 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q04609 (Residues: 44-750; Coverage: 94%)
Gene names: FOLH, FOLH1, GIG27, NAALAD1, PSM, PSMA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand NAG 3 x NAG
Ligand 29D 1 x 29D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I222
Unit cell:
a: 101.491Å b: 129.83Å c: 158.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.133 0.167
Expression system: Drosophila melanogaster

Quick links

Citations

3 review citations

Metal-Based PSMA Radioligands.
Gourni et al. (2017)
2 more

2 mentions without citation

Structural and biochemical characterization of the folyl-poly-γ-l-glutamate hydrolyzing activity of human glutamate carboxypeptidase II.
Navrátil et al. (2014)
1 more