› 4mcv

X-ray diffraction
2.73Å resolution

Star 12 bound to analog-sensitive Src kinase

Released:
DOI: 10.2210/pdb4mcv/pdb
PDB entry 4mcv coloured by chain, front view.
PDB entry 4mcv coloured by chain, side view.
PDB entry 4mcv coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Staurosporine-derived inhibitors broaden the scope of analog-sensitive kinase technology.
Lopez MS, Choy JW, Peters U, Sos ML, Morgan DO, Shokat KM
J Am Chem Soc 135 18153-9 (2013)
PMID: 24171479

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.83 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 256-533; Coverage: 52%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 29K 2 x 29K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 42.342Å b: 63.344Å c: 73.618Å
α: 100.34° β: 90.81° γ: 89.95°
R-values:
R R work R free
0.243 0.238 0.29
Expression system: Escherichia coli

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Citations

2 review citations

Exploiting holistic approaches to model specificity in protein phosphorylation.
Palmeri et al. (2014)
1 more