› 4mgk

X-ray diffraction
2.7Å resolution

Selective activation of Epac1 and Epac2

Released:
DOI: 10.2210/pdb4mgk/pdb
PDB entry 4mgk coloured by chain, front view.
PDB entry 4mgk coloured by chain, side view.
PDB entry 4mgk coloured by chain, top view.
Source organisms:
Entry author: Rehmann H

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-158239 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rap guanine nucleotide exchange factor 4 Chain: E
Molecule details ›
Chain: E
Length: 694 amino acids
Theoretical weight: 79.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EQZ6 (Residues: 324-1011; Coverage: 68%)
Gene names: Cgef2, Epac2, Rapgef4
Sequence domains:
Structure domains:
Ras-related protein Rap-1b Chain: R
Molecule details ›
Chain: R
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61224 (Residues: 1-167; Coverage: 91%)
Gene names: OK/SW-cl.11, RAP1B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand CMP 1 x CMP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I212121
Unit cell:
a: 125.04Å b: 144.55Å c: 227.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.264
Expression system: Escherichia coli

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